Lab Syllabus
| Week | Date | Activity | Preparatory Materials |
|---|---|---|---|
| 1 | Aug. 31–Sept. 4 | Lab orientation & overview handling flies (1) examine wild-type flies and begin life-cycle observations |
"In the Laboratory of Agassiz" |
| 2 | Sept. 7–11 | (2) plant genomic DNA prep (3) set up cross 1 for mutant mapping |
|
| 3 | Sept. 14–18 | (1) Life Cycle report due (2) run PCR reactions on plant DNA |
PCR protocol review PCR in our text (pp. 217–218) review PCR animations online (ask Prof. Google or refer to the "Study Aids" section on the course homepage) |
| 4 | Sept. 21–25 | (2) run agarose gel on PCR products (3) score mutant mapping cross 1 F1 progeny, set up F1 cross (3) set up mutant mapping crosses 2 & 3 for mutant mapping |
video on fly genetic nomenclature review pouring an agarose gel and gel electrophoresis; videos are available online from many sources, including benchfly, Bio-Rad (both pouring and running), and jove |
| Here is a link to pictures of the PCR gels; note that the red pixels visible on some images simply means that the signal was so strong there that it saturated the detector. | |||
| 5 | Sept. 28–Oct. 2 | DNA sequence analysis [Collier 206 Monday & Wednesday PPHAC 331 Thursday & Friday] |
|
| 6 | Oct. 5–9 | (2) sequence analysis (3) score mutant mapping cross 1 F2 progeny (3) score mutant mapping crosses 2 & 3 F1 progeny, set up F1 cross |
|
| 7 | Oct. 12–16 | (Fall Break) | |
| 8 | Oct. 19–23 | (2) Plant Identification report due (3) score mutant mapping crosses 2 & 3 F2 progeny (4) set up recombination mapping crosses |
|
| 9 | Oct. 26–30 | (5) purify human buccal cell DNA | |
| 10 | Nov. 2–6 | (3) Mutant Mapping report due (4)set up recombination mapping F1 crosses (5) run PCR on buccal cell DNA |
review the crossing schemes for crosses 2 and 3 review PCR basics |
| 11 | Nov. 9–13 | (5) run gel on buccal cell PCR products | |
| 12 | Nov. 16–20 | (4) score recombination mapping crosses progeny (5) Blood Genotyping report due |
|
| 13 | Nov. 23–27 | [no lab — Thanksgiving break] | |
| 14 | Nov. 30–Dec. 4 | (4) Recombination Mapping report due | |
| 15 | Dec. 7–11 | ||
Life is fluid, so this syllabus is subject to change. I don't anticipate any significant deviations, but remember that it's written in electrons, not stone.
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