abstracts

Jones, C.J., M. Homma, and R.M. Macnab. 1987. "Identification of Proteins of the Outer (L and P) Rings of the Flagellar Basal Body of Escherichia coli." Journal of Bacteriology 169(4):1489 1492.

Synthesis of the Salmonella typhimurium hook protein from the gene cloned on a multicopy plasmid results in partial suppression of the flagellar assembly defects of certain classes of Escherichia coli mutants (K. Ohnishi, M. Homma, K. Kutsukake, and T. Iino, J. Bacteriol., 169:1485-1488, 1987). This phenomenon allowed hook-basal body complexes from such mutants to be purified and analyzed by electron microscopy and gel electrophoresis. The absence of the P and L rings in such structures was found to correlate with the absence of proteins of apparent molecular weight 39,000 and 26,000, respectively. Gene-polypeptide correlations from other studies enabled us to complete gene-polypeptide-structure correspondences for these two proteins as flaM <—> 39-kilodalton protein <—> P ring and flaY <—> 26-kilodalton protein <—> L ring.